EMPIAR-10546

2.05 angstrom resolution structure determination of sulfur oxygenase reductase using 200kV cryo-EM

Publication:
Crystallographic and cryogenic electron microscopic structures and enzymatic characterization of sulfur oxygenase reductase from Sulfurisphaera tokodaii.
Sato Y , Yabuki T, Adachi N , Moriya T , Arakawa T , Kawasaki M , Yamada C , Senda T , Fushinobu S , Wakagi T
J Struct Biol X 4 (2020)
PMID: 32775998
Experiment type:
EMDB
Related PDB entry:
Related EMDB entries:
.EMD-30362 (2.05Å), EMD-30073 (2.24Å)
Deposited:
2020-11-03
Released:
2020-11-18
Last modified:
2020-11-18
Dataset size:
3.9 TB
Experimental metadata:
Contains:
micrographs
Image not available

Image set

1. 2.05 angstrom resolution structure determination of sulfur oxygenase reductase using 200kV cryo-EM
Category:micrographs - multiframe
Image format:MRC
No. of images or tilt series:2558
Frames per image:50
Image size:(4096, 4096)
Pixel type:SIGNED 16 BIT INTEGER
Pixel spacing:(0.68 Å, 0.68 Å)
Details:
.We started single particle analysis by using the nominal pixel size (0.69A/px). The pixel size was checked by comparing the cryo-EM structure with the atomic-coordinate model of the crystal structure using the 'Fit in Map' tool in UCSF Chimera and was finally calibrated to 0.676 A/pixel
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